基因组DNA (Genomic DNA)

产品描述

　　基因组DNA (Genomic DNA)

　　基因组DNA是一种确定分子检出限的理想工具，但若要更加有效，则需要在细胞系中提取出精确拷贝数及等位基因的gDNA，而这一切在患者细胞中并不容易获得。HDx参考标准品因其具有高度特色，其天然可用于生产DNA的细胞系成为理想的解决方案。

　　DNA参考标准品

　　Horizon的DNA标准品源自人工突变体(50%突变体)和匹配“正常”(0%突变体)的细胞系。突变细胞系都是杂合子，在大多数情况下，这意味存在着单个突变等位基因和单个正常等位基因。当拷贝数为2N时，100%的突变型DNA样品表明提取DNA的细胞中100%含有该突变，但由于该细胞的两个拷贝数中实际只有一个含有该突变，实际上该样本的等位基因突变频率为50%。

　　具有3N拷贝数和单个突变等位基因的基因型含有33%的等位基因突变频率。可以将突变体和“正常”DNA精确混合以产生具有特定等位基因频率的标准品。

　　图1. 使用基因组DNA来控制变异的流程图

　　产品列表：

产品货号	产品名称	突变频率	产品形式	规格
HD656	ABL1 T315I Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD657	ABL1 Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD659	AKT1 Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD658	AKT1 E17K Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD660	ALK F1174L Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD661	ALK Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD664	EML4-ALK Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD249	BRAF Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD238	BRAF V600E Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD239	BRAF V600K Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD248	BRAF V600R Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD246	BRAF V600M Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD244	BRAF V600G Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD253	EGFR G719S Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD254	EGFR L858R Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD257	EGFR L861Q Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD258	EGFR T790M Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD251	EGFR ΔE746-A750 Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD709	EGFR Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD260	EGFR V769_D770insASV Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD262	EGFR G719A Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD261	EGFR S768I Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD663	EGFR S492R Reference Standard	33%	Genomic DNA	1µg, 50ng/µl
HD665	FGFR2 S252W Reference Standard	33%	Genomic DNA	1µg, 50ng/µl
HD666	FGFR2 Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD667	FLT3 ΔI836 Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD668	FLT3 D835Y Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD669	FLT3 Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD670	GNA11 Q209L Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD671	GNA11 Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD672	GNAQ Q209L Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD673	GNAQ Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD674	GNAS R201C Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD675	GNAS Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD676	IDH1 R132C Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD677	IDH1 R132H Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD678	IDH1 Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD679	IDH2 R140Q Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD680	IDH2 R172K Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD681	IDH2 Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD652	JAK2 Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD649	JAK2 V617F Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD655	KIT Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD654	KIT D816V Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD265	KRAS G12A Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD269	KRAS G12C Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD272	KRAS G12D Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD287	KRAS G12R Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD288	KRAS G12S Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD289	KRAS G12V Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD290	KRAS G13D Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD757	NRAS A146T Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD299	KRAS A146T Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD758	KRAS K117N Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD291	KRAS Q61H Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD710	KRAS Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD694	KRAS A59T Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD298	KRAS Q61L Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD698	MEK1 P124L Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD699	MEK1 Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD682	MET Y1253D Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD683	MET Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD684	Notch1 L1600P Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD685	Notch1 Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD696	NRAS K117N Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD648	NRAS Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD303	NRAS Q61H Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD351	NRAS Q61K Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD412	NRAS Q61L Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD574	NRAS Q61R Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD697	NRAS A59T Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD302	NRAS G12V Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD695	NRAS G12D Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD686	PDGFRA D842V Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD687	PDGFRA Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD688	PI3KCA E542K Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD689	PI3KCA E545K Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD690	PI3KCA H1047R Genomic Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD691	PI3KCA Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD692	PTEN (Δex6/ex7) Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD693	PTEN Wild Type Reference Standard	100%	Genomic DNA	5µg, 50ng/µl
HD760	NRAS G13D Reference Standard	50%	Genomic DNA	1µg, 50ng/µl
HD701	Quantitative Multiplex Reference Standard		Genomic DNA	1µg, 50ng/µl
HD728	Tru-Q 1 (5% Tier) Reference Standard		Genomic DNA	1µg, 50ng/µl
HD729	Tru-Q 2 (5% Tier) Reference Standard		Genomic DNA	1µg, 50ng/µl
HD730	Tru-Q 3 (5% Tier) Reference Standard		Genomic DNA	1µg, 50ng/µl
HD731	Tru-Q 4 (5% Tier) Reference Standard		Genomic DNA	1µg, 50ng/µl
HD734	Tru-Q 7 (1.3% Tier) Reference Standard		Genomic DNA	1µg, 50ng/µl
HD802	EGFR Gene-Specific Multiplex Reference Standard		Genomic DNA	1µg, 50ng/µl
HD752	Tru-Q 0 (100% Wildtype) Reference Standard		Genomic DNA	1µg, 50ng/µl
HD753	Structural Multiplex Reference Standard		Genomic DNA	1µg, 50ng/µl
HD795	BRCA Somatic Multiplex		Genomic DNA	1µg, 50ng/µl
HD793	BRCA Germline I		Genomic DNA	1µg, 50ng/µl
HD794	BRCA Germline II		Genomic DNA	1µg, 50ng/µl